Insertion junction: CLIP2.012933_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.012933
Chromosome:chromosome 3
Location:5818896
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g187950 SEE1 Putative splicing endonuclease positive effector; (1 of 3) K10706 - senataxin [EC:3.6.4.-] (SETX, ALS4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTGAAGGCTACATCCTGCTGCCCTCCATCCATCCTGCAACCCTCGCT
Internal bar code:TCCCGGCTTAGGGACGCATGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:97
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGCGTGTGTGTGTTT
Suggested primer 2:AGCCGTCTCCACAAAGTGAG