| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.012988 |
| Chromosome: | chromosome 17 |
| Location: | 2818718 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718400 | (1 of 1) IPR001810//IPR011050 - F-box domain // Pectin lyase fold/virulence factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCTGCCACCTCGCAGTCCACCACACGCACCAGCGAGCCGCGCGCCTC |
| Internal bar code: | GTAAATATGAGGTGTGCTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1472 |
| LEAP-Seq percent confirming: | 16.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAAAGGAGAGCCCAGGAT |
| Suggested primer 2: | TCCTCGTCGTCCTCATCACT |