Insertion junction: CLIP2.013001_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.013001
Chromosome:chromosome 17
Location:3142981
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g720700 ANK14 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTGGGGAGGCGGCACAGCGCCAAACTAGGCCATCTTCTTTACCCCAA
Internal bar code:TAGAGCCACCCATTACGATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:240
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTAAACACGACCCCTTGA
Suggested primer 2:CATTGCTCACGTTGGGGTTG