Insertion junction: CLIP2.013014_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013014
Chromosome:chromosome 12
Location:4637418
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g522150 FAP194 (1 of 239) IPR016024 - Armadillo-type fold; Flagellar Associated Protein 194 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACACTGAATGGCTGCGCTGCGCGCCACTCACTGTCCATGACCAGGGGC
Internal bar code:GGCCATACGGTCAGGAAAAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2310
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TACCTGGCGATGGAGAAGGA
Suggested primer 2:TCTACTACCACCGCCACAGA