Insertion junction: CLIP2.013024_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.013024
Chromosome:chromosome 17
Location:1239359
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g705050 LIC1 (1 of 3) PF02931//PF02932 - Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD) // Neurotransmitter-gated ion-channel transmembrane region (Neur_chan_memb); Ligand-gated ion-channel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGCGCCGCCGGCAAAGGCGGCGCCAGCCGCCACCGTGGCTGCTGCGG
Internal bar code:TAGATGCGCGTGCGGCCAAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1154
LEAP-Seq percent confirming:41.6667
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGAGAGCGTGTGGACACT
Suggested primer 2:CGTGCGACTCTACGAGTACC