| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013036 |
| Chromosome: | chromosome 1 |
| Location: | 2684334 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016200 | MLO4 | (1 of 5) PF03094 - Mlo family (Mlo); MLO family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGGATCCTAGGGCGCCACAGGAGGAGGCTGGGCGCGAGAGGTGGCCG |
| Internal bar code: | GCGGTGTGTAAAGAGAAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGTTGCACCAACTCAAG |
| Suggested primer 2: | GTGGTGGGAAGACAGGAGTG |