| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013042 |
| Chromosome: | chromosome 16 |
| Location: | 1786542 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655100 | (1 of 1) PF04266 - ASCH domain (ASCH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAACGGTACCCTAGTTGCGGGGGCGGGGTCAGCGTCGGGGCGAGGACCA |
| Internal bar code: | GAGCTATACCTACACTGCATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1833 |
| LEAP-Seq percent confirming: | 88.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCGTTACACGTGTAAGA |
| Suggested primer 2: | TGCGTGAGATGCCAGACATT |