| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.013043 |
| Chromosome: | chromosome 4 |
| Location: | 1169321 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215600 | (1 of 1) PTHR32183:SF6 - THIOCYANATE METHYLTRANSFERASE 1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGATGAAACAGTGCCTTGGTTGCGAAAATGTGCACGAAAAGCAACTTT |
| Internal bar code: | TTGGCATAATGGTAAATCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3175 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTGTACGGAGGTGAAA |
| Suggested primer 2: | CATTTTGAGCAAACGCCCCA |