Insertion junction: CLIP2.013082_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.013082
Chromosome:chromosome 13
Location:2966623
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g583750 (1 of 37) PF13639 - Ring finger domain (zf-RING_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGTGCTTTGATGAGTTGGGGGCTGAGTCCGCGTCATCAGAGGCGCTG
Internal bar code:GTTGGCAGTGTCAAGGGTAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:947
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGTAAACGCCCACGAGTT
Suggested primer 2:TAATTCACTCTGCGCCGTGT