| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.013088 |
| Chromosome: | chromosome 17 |
| Location: | 2065777 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711250 | MIP2 | (1 of 2) PTHR19139:SF180 - AQUAPORIN-1; Aquaporin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCGCATGTGCACACGCATGGGGAGGGCCGGAGGAGGGACGGGGCGG |
| Internal bar code: | CGCGCACGTAAGATAAACTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCCCTTGTTGCAGAGTG |
| Suggested primer 2: | CCTGTCATACCCACACCCAC |