Insertion junction: CLIP2.013090_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013090
Chromosome:chromosome 3
Location:4605609
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g176700 mS29,MRPS29,CPL4 Mitochondrial ribosomal protein S29; (1 of 1) K17408 - small subunit ribosomal protein S29 (DAP3, MRPS29) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCGCTGGTCCTAGCGCTCCCACCACTTACCACATCCACTCGCCTCCG
Internal bar code:AATAGGTCTGCGGTCTAACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1566
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACCAGCCTGATGGATTG
Suggested primer 2:GGTCGCGTAGGGGTTCATAG