Insertion junction: CLIP2.013091_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013091
Chromosome:chromosome 6
Location:1794089
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g263289 (1 of 1) 2.5.1.87 - Ditrans,polycis-polyprenyl diphosphate synthase ((2E,6E)-farnesyl diphosphate specific) / Rer2p Z-prenyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATATTGGGTTACTGCACGCAAGCTCCCACGGAGTAGGGGTCTTGCAAAA
Internal bar code:CTAATTCCGGTCCAGGGCATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2570
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGACAGCATTCGTATCGA
Suggested primer 2:GCAGGGTGTGGGGTTGATTA