| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.013107 |
| Chromosome: | chromosome 12 |
| Location: | 5533060 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530550 | KDG2 | (1 of 1) 2.7.1.91 - Sphinganine kinase / Dihydrosphingosine kinase; Diacylglycerol kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGAAGCGCAGCTCGCCCATCCACCTGGCGACGCGGAGGAAGCAACAG |
| Internal bar code: | TGAGCGTTTTTCTATGCATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2301 |
| LEAP-Seq percent confirming: | 93.4426 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGATGTTGAGGCTGTGGG |
| Suggested primer 2: | CCAAAAGACGCACATGCACA |