| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.013109 |
| Chromosome: | chromosome 1 |
| Location: | 2690780 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016250 | MLO3,MLO4 | (1 of 5) PF03094 - Mlo family (Mlo); MLO family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATGCGGTTCGCGGGCGGGCCCCCTCACTGCCCTCCCCGCCGCAGCCT |
| Internal bar code: | TGCGTTCTCGAGCCGGGGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2334 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGCTTACAGTGGGATTGG |
| Suggested primer 2: | AAGCTCTTATTGTCCGGGCC |