| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013137 |
| Chromosome: | chromosome 9 |
| Location: | 1540225 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399250 | KIR1 | (1 of 7) PF12680 - SnoaL-like domain (SnoaL_2); Ketoacid isomerase-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCCGTAAGTACCGTACGGTTTCTAACGTACGGGACGCCAAGCGACCG |
| Internal bar code: | TGTTGTAAATTAGGGACACCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATGCACAAAAGTCCGCA |
| Suggested primer 2: | TTGTGTCCAGTGGCGACTAC |