| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013149 |
| Chromosome: | chromosome 12 |
| Location: | 1781807 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499301 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGCGTGGATTGAGGCACAGCAAGATTCACAAACAGTCGATTACACC |
| Internal bar code: | CGGGCGCGAATACTGGATTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1846 |
| LEAP-Seq percent confirming: | 80.9524 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGTGTAGAGAGTCGTCT |
| Suggested primer 2: | GGTCTTGACTTCCTGCGGAA |