Insertion junction: CLIP2.013151_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013151
Chromosome:chromosome 2
Location:804323
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g078550 FAP210 (1 of 5) PF13868 - Trichohyalin-plectin-homology domain (TPH); Flagellar Associated Protein 210 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGGCGGCCTTGCCAAACCCCCGTCCCCACGCCTTTGGCGCACACTCC
Internal bar code:TTAAGTTCTAAACAGTCGTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1922
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCACCTCTACTACCCCA
Suggested primer 2:GCGCCTAAACGAATGCAGAG