Insertion junction: CLIP2.013161_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.013161
Chromosome:chromosome 10
Location:4746894
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g452550 HOPP1,HOP1 Homologous-pairing protein 1; (1 of 3) PF02301 - HORMA domain (HORMA) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCTATCCACCCCATGCCCCCAGAGGCACAGTCTGTACGCAACATCGC
Internal bar code:TGAGAAGGTAGAGAGAGGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1152
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAGAACCCTCCTCGCTAT
Suggested primer 2:CAAGGGGTTTTGCTTGGCTC