Insertion junction: CLIP2.013177_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.013177
Chromosome:chromosome 1
Location:6760255
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g048300 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTGGAAGCCGTCTAGACTGAAGCTGCGCGAATACAACGACTCCTCATC
Internal bar code:TTATGCGACATTTAAAACCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1817
LEAP-Seq percent confirming:41.9355
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCACAACCCGATTAACCC
Suggested primer 2:AACATCTCCTCCAACGCCAG