Insertion junction: CLIP2.013188_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013188
Chromosome:chromosome 6
Location:8876345
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g311700 FAP349 Leucine-Rich Repeat Flagellar Associated Protein 349 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGCAGCTCCAAAGCTTTACGGTGGTAAGTTCGATAACTGAGGAGATTG
Internal bar code:GCCGAGACGGTATTGGAACTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1996
LEAP-Seq percent confirming:97.3684
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CATTGGCCACCCATACCCTT
Suggested primer 2:CGCGCATGATTTGAGACTGG