Insertion junction: CLIP2.013190_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.013190
Chromosome:chromosome 2
Location:6057352
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g119050 CER1 Carboxyesterase-related protein; (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGAATCGTGCGCGGGCTTGTGGCCCGCGCCATCGCCTGCTTGGTGCT
Internal bar code:GACCTAGTCTTCAAAAATTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:3338
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCAGATATTTGGCGCCAG
Suggested primer 2:GGTAATAGAGGTCCAGGCGC