Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.013194 |
Chromosome: | chromosome 6 |
Location: | 73066 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g249350 | DMC3,DMT1A | (1 of 4) K00558 - DNA (cytosine-5)-methyltransferase 1 (DNMT1, dcm); Putative DNA methylase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCGTCCGTGGCCGAGCGGGATGAGCCGGTGGTGGTGGTGGTGATGG |
Internal bar code: | GTTGATCTTTGTCCTGGATTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2483 |
LEAP-Seq percent confirming: | 35.7143 |
LEAP-Seq n confirming: | 20 |
LEAP-Seq n nonconfirming: | 36 |
LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTCCAAGTACTGCGTGGTG |
Suggested primer 2: | CAAGCACGCAAAGCAAGACT |