Insertion junction: CLIP2.013229_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.013229
Chromosome:chromosome 3
Location:1325053
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g150450 (1 of 11) IPR023296 - Glycosyl hydrolase, five-bladed beta-propellor domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGTGACGGGCAACATATATGTGCTCTTTGCACGCGTCTACGTCGCGAA
Internal bar code:CGTCTAACCCCTGCTCCTTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1129
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGCATGTGCAGGCAGTTA
Suggested primer 2:TGAGTAAGGCGACACCCCTA