Insertion junction: CLIP2.013242_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.013242
Chromosome:chromosome 3
Location:1251114
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g149800 TOG1 (1 of 1) K16803 - cytoskeleton-associated protein 5 (CKAP5, XMAP215); Microtubule associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCTCCCGAGTGACCGCCCGCATCACGCGCCGGCTGACTCACCCGCGC
Internal bar code:CCGTAACACCGATAGTCGCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1918
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTCTTGCTGACCTGGTGC
Suggested primer 2:TTGGCTTCAGTGCACCAGAA