Insertion junction: CLIP2.013250_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013250
Chromosome:chromosome 17
Location:2666676
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g716950 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACCCGGGTGGCCCCCACCCGAGTGACCCCCGCCGCGGCCGCTGCTGC
Internal bar code:CCATGGTTCGCGGCTTAACAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1307
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGACCTCAACACGACCTCAA
Suggested primer 2:TTTCCAGGACCAACCGTACG