| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.013267 |
| Chromosome: | chromosome 4 |
| Location: | 1893061 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218300 | (1 of 1) K14774 - U3 small nucleolar RNA-associated protein 25 (UTP25, DEF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCAGGACGGCCACCCCCTCCTTTCCCCTCCTCCTCGGCCGCCACCTG |
| Internal bar code: | TATTGAGCACAAGTTCGCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGAAGGAGGAGGAGCAG |
| Suggested primer 2: | GTAACCTTGATGCCCAGCCT |