Insertion junction: CLIP2.013277_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.013277
Chromosome:chromosome 2
Location:2785549
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094150 HIK,HIK2,HKR2 (1 of 1) K12130 - pseudo-response regulator 5 (PRR5); Response regulator of two-component system CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATTTCCAGAAGAAGGTGAGCGCCGATACATGAGCGCTAAGTAGATGCA
Internal bar code:TTGTAGGGTCTATCTGAATCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2103
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTTCGCCATCGTCCTCAT
Suggested primer 2:TCGCTTTCTGTGCTACCTCC