| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.013296 |
| Chromosome: | chromosome 2 |
| Location: | 1681342 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085750 | (1 of 8) PF13416 - Bacterial extracellular solute-binding protein (SBP_bac_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTAAACAATGTATGCAGGCGTTACGAGACCGAGCGGGATACCGAAACA |
| Internal bar code: | CTCTATGCAGCTTCCATTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2712 |
| LEAP-Seq percent confirming: | 98.5507 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAAACACAGGCTGGACA |
| Suggested primer 2: | AAGTGGGGCTTCCTACATGC |