| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.013296 |
| Chromosome: | chromosome 2 |
| Location: | 1681389 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085750 | (1 of 8) PF13416 - Bacterial extracellular solute-binding protein (SBP_bac_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATGGAGGCGGCCCTGCGGGTAAAGCTGGGTTTGTGCAAGCTTGGACA |
| Internal bar code: | TTAATTGCAGAGTAAATGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1882 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGGGGCTTCCTACATGC |
| Suggested primer 2: | ACACAAACACAGGCTGGACA |