| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.013316 |
| Chromosome: | chromosome 1 |
| Location: | 1976747 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010880 | (1 of 2) K18598 - echinoderm microtubule-associated protein-like 6 (EML6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACACAAGCACACACACACGCTCGATCCCTGGCGACCGTGCCCCTGGA |
| Internal bar code: | CACTCACTGTAGAGCTATTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAGAAGGACGACAAGGG |
| Suggested primer 2: | CCACCCTTTCGTGTTCCGTA |