Insertion junction: CLIP2.013321_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.013321
Chromosome:chromosome 9
Location:2691328
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGATTTCATCACGCTTGCGAATGGGAATGGGCATTCATGTGCGAGGTA
Internal bar code:ATATGATGGCGCGGCAGCATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2339
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCCACAGACACACGTAA
Suggested primer 2:TAAACCTGACGTGTTCGCGA