| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.013367 |
| Chromosome: | chromosome 12 |
| Location: | 7418006 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557250 | (1 of 15) 3.6.5.5 - Dynamin GTPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGAGACGCTATCCACCATGGAGCACTAAGCGGCTTGGCAGGAGGAGG |
| Internal bar code: | TTATATATGGCTTAATCCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4243 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCATACCCTGAAGGCAC |
| Suggested primer 2: | TATTGCATTGTAGCGCACGC |