Insertion junction: CLIP2.013371_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.013371
Chromosome:chromosome 16
Location:1669546
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g654600 PHC56 (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCGCCACAAAAGGCCGCCGGCAGCCGCCCCAGCCACTCGGCCACCA
Internal bar code:ACGGAGACAGAAATCAACCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:505
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:ATACAGCAGTAGCCGCCATC
Suggested primer 2:ACACACACACACACACACCT