Insertion junction: CLIP2.013371_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.013371
Chromosome:chromosome 16
Location:1669555
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g654600 PHC56 (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGAGGGGTTTGCGAGTGCTGGTAACCCTGATGCCCCCCGAGCTCTCT
Internal bar code:ACGGAGACAGAAATCAACCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3649
LEAP-Seq percent confirming:98.0198
LEAP-Seq n confirming:99
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:101

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACCT
Suggested primer 2:ATACAGCAGTAGCCGCCATC