Insertion junction: CLIP2.013386_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.013386
Chromosome:chromosome 5
Location:980320
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g247050 CTR4,COPT1 CTR type copper ion transporter; (1 of 2) K14686 - solute carrier family 31 (copper transporter), member 1 (SLC31A1, CTR1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGACCATGCCGCGCACAGCCACGGCGCTAGCAACACGCCCGCTCCATCC
Internal bar code:GCCAGCAACACCGTAGTACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:84.375
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCGCCACTCACTTCTGTC
Suggested primer 2:CAAGAAGTTCGCAAGTGCCC