Insertion junction: CLIP2.013390_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013390
Chromosome:chromosome 7
Location:1475391
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g324000 NUP160 Nucleoporin 160; (1 of 54) IPR011047 - Quinonprotein alcohol dehydrogenase-like superfamily CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCGCCGTCGCCGGACGTGACCGCCTCTGGCCCAAGCTGCGGACAGGG
Internal bar code:CGATCGTTAACGATCATATTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:430
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCTCGTCCTTGTGGCATG
Suggested primer 2:GTGTGACATCTGACGCATGC