Insertion junction: CLIP2.013397_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013397
Chromosome:chromosome 5
Location:151816
Confidence (%):80
Locus systematic id Locus common name Defline Feature
lncRNA_TCONS_00048232

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCTTACACGGCGTTGACACCACGCGCCCCACAGGCGATTGGTAGGCA
Internal bar code:AAATCAATATAGAGAAGTGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3614
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GATCCACGGGCGAACAAATG
Suggested primer 2:TGTTGCCCATCATCGTCACA