Insertion junction: CLIP2.013430_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013430
Chromosome:chromosome 11
Location:2661203
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095076 ROC39,MFT10 Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGACAACACCTCGACGCAAGTCCTGCTCAGTGAGAGACTGAGACTCCT
Internal bar code:GTATTTTTGCTTTGGCGGGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2716
LEAP-Seq percent confirming:96.2025
LEAP-Seq n confirming:76
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCGAGGAGACGAACGAAG
Suggested primer 2:GAACATGTCCGCGATGATGC