| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.013435 |
| Chromosome: | chromosome 13 |
| Location: | 1350504 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571500 | CST2 | Chlamydomonas-specific membrane transporter of unknown function | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGGTAGAGTGCGAAAGCATAAAGATCGGTTGCGCAGTGTTTTCGCC |
| Internal bar code: | GAATGCTTATGTCATGTGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCAGCTTGTCACTCCCG |
| Suggested primer 2: | GCAAAGGGACAAAAGGGCAG |