Insertion junction: CLIP2.013453_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.013453
Chromosome:chromosome 16
Location:3588697
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670261 (1 of 1) K15305 - vacuole morphology and inheritance protein 14 (VAC14, TAX1BP2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGCCAACGCTGCGCTGCTGCGGCTCGAGGTGCGCGTGTCTCGACCGG
Internal bar code:CGACGACGTAGTTCCAACACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1015
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGTGAGTTCCAGACAGAA
Suggested primer 2:GGAAAAGAACACAACGGGGC