| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.013458 |
| Chromosome: | chromosome 16 |
| Location: | 1333868 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651750 | TSI,TSI2 | isoleucyl-tRNA synthetase; (1 of 2) K01870 - isoleucyl-tRNA synthetase (IARS, ileS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCACACTCGCGCGGCGGCCGATGATATTACACACATTGTTACACACG |
| Internal bar code: | TCCCTTTACTTTGTTGGGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1621 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGGGCAGGAAAACGTC |
| Suggested primer 2: | TAGTTGCTTGGCCACGACAT |