Insertion junction: CLIP2.013464_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013464
Chromosome:chromosome 13
Location:1348363
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g571450 (1 of 2) K17278 - membrane-associated progesterone receptor component (PGRMC1_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTACCCCACCCCGACTCCCACCACACCCGGTAACTGTTCACCGCCGTAT
Internal bar code:TGAGCATCGATCAGGATGAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1751
LEAP-Seq percent confirming:82.8571
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCAAAATGAACCGCCCG
Suggested primer 2:CCGATACTCTTTAGGCGCGT