| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.013466 |
| Chromosome: | chromosome 3 |
| Location: | 2962519 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163000 | POC5 | (1 of 1) K16483 - centrosomal protein POC5 (POC5); Proteome of centriole protein 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTATCCCTTGGCGGCGGCAGCAGCATAGACATCCTGGTGCCGGCAGCAG |
| Internal bar code: | TATTGCAACTGGCGATGCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1602 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTTGAGCAGGTTGATCT |
| Suggested primer 2: | AGTCTCTGCGAAAGTCCTGC |