| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.013483 |
| Chromosome: | chromosome 7 |
| Location: | 3281718 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334450 | EFH4 | EF-hand domain protein; (1 of 1) IPR007044 - Cyclodeaminase/cyclohydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGGATTTGCCCCGCTCCCAAAGCCCGCCAGCGCCTGCTGTTCTGCCT |
| Internal bar code: | TATATAATGTGAATTTGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4373 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATGGTACTGCGGTGCAAG |
| Suggested primer 2: | GCCACCTCCGTCACCTAAAA |