| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.013562 |
| Chromosome: | chromosome 6 |
| Location: | 7324861 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299500 | intron | ||
| Cre06.g299550 | ZIP6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCCCTGCTACACACTCCCACGCCCTCACTCACCCTCACTCACCCAGG |
| Internal bar code: | TTATTGGGAAAAGGGCGCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATAGCGTGTGCCTGCATG |
| Suggested primer 2: | TCTCCGAACGTTGCCATGAA |