| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.013569 |
| Chromosome: | chromosome 10 |
| Location: | 2200710 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433600 | (1 of 1) 1.5.1.20 - Methylenetetrahydrofolate reductase (NAD(P)H) / N(5,10)-methylenetetrahydrofolate reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCCCATGCGAGTGTCCTACAACGTTCCCAGGCACTCCTAACCCGAAT |
| Internal bar code: | TGTTGTCAGGGGGGAGCACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGCAGGTACATCAAGGG |
| Suggested primer 2: | GTTGTGTCTGGGTAGTGCGA |