Insertion junction: CLIP2.013572_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.013572
Chromosome:chromosome 6
Location:8207885
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g306200 CDS
Cre06.g306250 Hypothetical protein; (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACCATGTGGGCCGACAGCACGTCGGCATGGATGTACCTTACGGACCT
Internal bar code:TGGTAGTTGGGCTGCTGTATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1066
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGGAGAGGGGTAAGAGAA
Suggested primer 2:CGTCTGCAACAATAGCTCGC