Insertion junction: CLIP2.013576_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.013576
Chromosome:chromosome 2
Location:6006032
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118600 FTSZ3,FTSZ1 (1 of 2) 3.6.5.6 - Tubulin GTPase; Plastid division protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATCATCCGCCTTCCGGCTGAACGCAGACCATCCAGATGCCGTCACATG
Internal bar code:ACAGCAGTTCTTGTGTGTGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2287
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGACGATCACGGAGTCC
Suggested primer 2:TATATGGTCGTCGCCAGCAC