Insertion junction: CLIP2.013577_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.013577
Chromosome:chromosome 17
Location:1563794
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g707150 LIC2 Hypothetical protein; (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTCCACCACCATGTCGTACCACTGCACCACCACCGCATCACTCACCAA
Internal bar code:GAATGGTAGGTGGGGACAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2033
LEAP-Seq percent confirming:94.5946
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGGACGATGAAGATTGC
Suggested primer 2:GAGGGTAAGAGGGAGAGGCA