Insertion junction: CLIP2.013580_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.013580
Chromosome:chromosome 4
Location:1722173
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g212200 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGCCCAGGTGGAGGTGACGACTACTGCCCCAACGCGACAGACATGATG
Internal bar code:CGAAATATTCGGGGCTTATGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1196
LEAP-Seq percent confirming:70.5882
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTCTGCTGACGTGTTGC
Suggested primer 2:CAGACACCGCTTTCAGCAAC